A novel multi-pad paper plate (MP) based assays for rapid animal disease diagnostics
نویسندگان
چکیده
This study describes a novel fabrication method of a multi-pad paper plate (MP) for transfer and optimisation of enzyme-linked immunosorbent assays (ELISA) on paper. The plate was fabricated using a combination of laser cutting and lamination for rapid and low-cost production of a multi-pad paper plate compatible with a standard 96 well microplate format. The plate was used to transfer a sandwich ELISA assay for detection of the acute phase protein bovine haptoglobin, allowing assay optimisation in the new format. The optimised protocol was also applied to detection of haptoglobin in bovine serum, demonstrating significant correlation with the traditional lab-based ELISA. © 2016 The Authors. Published by Elsevier Ltd. Peer-review under responsibility of the organizing committee of the 30th Eurosensors Conference.
منابع مشابه
Two in-House One-Step rRT-PCR Assays, Developed for Accurate and Rapid Molecular Identification of Newcastle Disease Virus, on the basis of SYBR Green and Specific TaqMan Probe
Background and Aims: Newcastle disease virus (NDV) is an avian paramyxovirus (A-PMV 1) and one of the major pathogens in poultries. Vaccination is intended to control the disease, nevertheless this virus is a growing threat to the poultry industry. So, early detection of the virus can prevent the spread of illness and avoid huge economic losses. Towards this goal, in this research, we develop...
متن کاملDevelopment of antibody-based microarray assay for quantitative detection of aflatoxin B1
BACKGROUND: Aflatoxin B1 (AFB1) is a toxic metaboliteproduced by Aspergillus species that contaminates a wide range ofagricultural products. OBJECTIVES: This study was designed todevelop a rapid and highly sensitive immunoassay method inmicroarray format for quantitative detection of AFB1 to evaluatethe potential of microarray platform for high-throughput screening,which can be beneficial in fo...
متن کاملMulti-perspective Decision Support System for Hierarchical Bus Transportation Network Design: Tehran Case Study
In this paper, a multi-perspective decision support system (MP-DSS) to design hierarchical public transportation network is developed. Since this problem depends on different perspectives, MP-DSS consists of two sub-systems with macro and micro sub-systems based on travel information, land use and expert knowledge. In the micro sub-system, two sub-modules are developed considering o...
متن کاملCOVID-19; History, Taxonomy, and Diagnostic Molecular and Immunological Techniques
In recent years, viral epidemics such as the coronavirus disease (COVID-19) have spread, and this outbreak is thought to be the result of animal-to-human transmission. Hence, accurate diagnostic tests to detect COVID-19 and antiviral antibodies in infected individuals are of utmost importance. This report describes the structure, history, taxonomy, and molecular and immunological techniques for...
متن کاملAdvances in paper-based point-of-care diagnostics.
Advanced diagnostic technologies, such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA), have been widely used in well-equipped laboratories. However, they are not affordable or accessible in resource-limited settings due to the lack of basic infrastructure and/or trained operators. Paper-based diagnostic technologies are affordable, user-friendly, rapid, robust,...
متن کامل